1. Reagents should be brought to room temperature, 20–25ºC prior to use. Avoid prolonged (> 24 hours) storage at room temperature. 2. Immediately start the next step of operation after the washing procedure, avoid the microwell be dry affecting the detecting result. 3. Shake the reagent before use. 4. The Stop Solution is 2M sulphuric acid. Avoid contact with skin and mucous membranes. Immediately clean up any spills and wash area with copious amounts of water. If contact should occur, immediately flush with copious amounts of water. 5. Do not use reagents after expiration date. Do not substitute reagents from any other manufacturer into the test kit. Do not combine reagents from other Mycotoxin ELISA Kits with different Lot numbers. 6. The kit should be store at the temperature 2-8℃, do not freeze. Be sure to re-seal unused wells in the zip-lock bag with desiccant stored. Avoid directly exposure the reagents to light. 7. Any color conversion indicates that the reagent is deteriorated, please drop it. If the absorbance of negative control absorbance is less than 0.5, the reagent is probably deteriorated. 8. The incubating time is usually 15-20 minutes after dispensing Substrate A and 50µL Substrate B into the wells. If the color too less, please prolong the incubating time but should not exceed 30 minutes. Conversely, reduce the incubating time. 9. Crystallization of the Concentrated Wash buffer is a normal phenomenon, heat it before using. 10. The optimum reaction temperature is 25℃, too high or two low temperature would arise the change of the absorbance and sensitivity.